LOCUS       40924_27099        7254 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKU1021fps DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Yamada Y, Kuzuyama T, Komatsu M, Shin-Ya K, Omura S, Cane DE, Ikeda 
            H.
  TITLE     Terpene synthases are widely distributed in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (3), 857-862 (2015)
  PUBMED    25535391
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Ikeda H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2014) Contact:Haruo Ikeda Kitasato University, 
            Kitasato Institute for Life Sciences; 1-15-1 Kitasato, Sagamihara, 
            Kanagawa 252-0373, Japan URL :http://avermitilis.ls.kitasato-u.ac.jp
REFERENCE   3  (bases 1 to 7254)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7254)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "3"; pages: 
            "857-862"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2014) Contact:Haruo Ikeda Kitasato University, Kitasato 
            Institute for Life Sciences"; volume: " 1-15-1 Kitasato, Sagamihara,
            Kanagawa 252-0373, Japan URL :http"; pages: 
            "//avermitilis.ls.kitasato-u.ac.j"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             353..1144
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(1485..1594)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1648..1659)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    1730..1829
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             1839..3653
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
     rep_origin      3876..4464
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4752..4773
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4788..4818
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4826..4842
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4850..4866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4909..5922)
                     /codon_start=1
                     /gene="ptlB"
                     /product="farnesyl diphosphate synthase"
                     /label=ptlB
                     /note="from Streptomyces avermitilis"
                     /protein_id="BAP82237.1"
                     /translation="MTVTPESLHRSRDAITPALRTALHLLDEETRHQAAYHLGWIEADG
                     TPADGGGGKAVRPALALLSAQAAGAPAETGLPGAVAVELVHNFSLLHDDLMDGDEERRH
                     RRTVWTLWGASGAILTGDALLALAQEVLLDCGLPTGAAAARLLARTTRDLIRGQVQDVA
                     FERRAHVPIEECVDMAAGKTGALLSASAAIGAVLAGAPVTTVDALALYGQQVGLAFQLT
                     DDVLGIWGDPAVTGKSVHSDLRARKKSLPVTYALNQGGTVSVELATWLSAPGEPDEAEL
                     RRAADLVAAAGGRDWATAEAARRMALGEAALRGVDLDAAARDELIALGHFVVDREV"
     gene            complement(4909..5922)
                     /gene="ptlB"
                     /label=ptlB
     regulatory      complement(5926..6472)
                     /gene="rpsJp"
                     /note="promoter of gene encoding ribosomal protein S10;
                     from Streptomyces avermitilis MA-4680"
                     /regulatory_class="promoter"
     gene            complement(5926..6472)
                     /gene="rpsJp"
                     /label=rpsJp
     promoter        6477..6505
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6694..7224
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
       61 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
      121 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
      181 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
      241 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
      301 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
      361 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
      421 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
      481 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tccaagacga
      541 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
      601 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
      661 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
      721 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
      781 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
      841 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cggatgcccg acggcgagga
      901 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
      961 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     1021 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     1081 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     1141 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     1201 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     1261 gacgccctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac
     1321 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct
     1381 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt
     1441 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
     1501 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     1561 gctcgcgggt gggcctactt cacctatcct gccccgctga cgccgttctc gagcgtgccg
     1621 gagcaatcgc cctgggtggg ttacacgacg cccctctatg gcccgtactg acggacacac
     1681 cgaagccccg gcggcaaccc tcagcggatg ccccggggct tcacgttttc ccaggtcaga
     1741 agcggttttc gggagtagtg ccccaactgg ggtaaccttt gagttctctc agttgggggc
     1801 gtagggtcgc cgacatgaca caaggggttg tgaccggggt ggacacgtac gcgggtgctt
     1861 acgaccgtca gtcgcgcgag cgcgagaact cgagcgcagc aagcccagcg acacagcgta
     1921 gcgccaacga agacaaggcg gccgaccttc agcgcgaagt cgagcgcgac gggggccggt
     1981 tcaggttcgt cgggcatttc agcgaagcgc cgggcacgtc ggcgttcggg acggcggagc
     2041 gcccggagtt cgaacgcatc ctgaacgaat gccgcgccgg gcggctcaac atgatcattg
     2101 tctatgacgt gtcgcgcttc tcgcgcctga aggtcatgga cgcgattccg attgtctcgg
     2161 aattgctcgc cctgggcgtg acgattgttt ccactcagga aggcgtcttc cggcagggaa
     2221 acgtcatgga cctgattcac ctgattatgc ggctcgacgc gtcgcacaaa gaatcttcgc
     2281 tgaagtcggc gaagattctc gacacgaaga accttcagcg cgaattgggc gggtacgtcg
     2341 gcgggaaggc gccttacggc ttcgagcttg tttcggagac gaaggagatc acgcgcaacg
     2401 gccgaatggt caatgtcgtc atcaacaagc tcgcgcactc gaccactccc cttaccggac
     2461 ccttcgagtt cgagcccgac gtaatccggt ggtggtggcg tgagatcaag acgcacaaac
     2521 accttccctt caagccgggc agtcaagccg ccattcaccc gggcagcatc acggggcttt
     2581 gtaagcgcat ggacgctgac gccgtgccga cccggggcga gacgattggg aagaagaccg
     2641 cttcaagcgc ctgggacccg gcaaccgtta tgcgaatcct tcgggacccg cgtattgcgg
     2701 gcttcgccgc tgaggtgatc tacaagaaga agccggacgg cacgccgacc acgaagattg
     2761 agggttaccg cattcagcgc gacccgatca cgctccggcc ggtcgagctt gattgcggac
     2821 cgatcatcga gcccgctgag tggtatgagc ttcaggcgtg gttggacggc agggggcgcg
     2881 gcaaggggct ttcccggggg caagccattc tgtccgccat ggacaagctg tactgcgagt
     2941 gtggcgccgt catgacttcg aagcgcgggg aagaatcgat caaggactct taccgctgcc
     3001 gtcgccggaa ggtggtcgac ccgtccgcac ctgggcagca cgaaggcacg tgcaacgtca
     3061 gcatggcggc actcgacaag ttcgttgcgg aacgcatctt caacaagatc aggcacgccg
     3121 aaggcgacga agagacgttg gcgcttctgt gggaagccgc ccgacgcttc ggcaagctca
     3181 ctgaggcgcc tgagaagagc ggcgaacggg cgaaccttgt tgcggagcgc gccgacgccc
     3241 tgaacgccct tgaagagctg tacgaagacc gcgcggcagg cgcgtacgac ggacccgttg
     3301 gcaggaagca cttccggaag caacaggcag cgctgacgct ccggcagcaa ggggcggaag
     3361 agcggcttgc cgaacttgaa gccgccgaag ccccgaagct cccccttgac caatggttcc
     3421 ccgaagacgc cgacgctgac ccgaccggcc ctaagtcgtg gtgggggcgc gcgtcagtag
     3481 acgacaagcg cgtgttcgtc gggctcttcg tagacaagat cgttgtcacg aagtcgacta
     3541 cgggcagggg gcagggaacg cccatcgaga agcgcgcttc gatcacgtgg gcgaagccgc
     3601 cgaccgacga cgacgaagac gacgcccagg acggcacgga agacgtagcg gcgtagcgag
     3661 acacccggga agcctgttag gcgctgagac gggcgcacag cgggcttcct ggggcaatgc
     3721 atcctccagc gcggggatct catgctggag ttcttcgccc accccaaaag gatctaggtg
     3781 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3841 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3901 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3961 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4021 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4081 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4141 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4201 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4261 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4321 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4381 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4441 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4501 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4561 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4621 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4681 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4741 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     4801 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     4861 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagact acacctcccg
     4921 gtcgacgacg aagtgtccga gggcgatgag ctcgtcgcgc gccgcggcgt ccaggtcgac
     4981 gccgcgcagc gcggcctccc cgagcgccat gcgccgggcc gcctcggcgg tggcccagtc
     5041 gcggccgccg gccgccgcca ccaggtcggc cgcccggcgc agctctgcct cgtcgggctc
     5101 gcctggtgcg gagagccagg tggccagctc gacactcacg gtcccgccct ggttgagcgc
     5161 gtacgtgacc ggcagcgact tcttgcgggc ccgcaggtcg gagtggaccg acttgccggt
     5221 gacagccggg tcgccccaga tgccgaggac gtcgtcggtc agctggaagg cgaggcccac
     5281 ctgctgcccg tagagcgcca gggcgtcgac ggtggtcacc ggcgcgccgg ccagtacggc
     5341 accgatcgcg gcgctggccg acagcagcgc gccggtcttg ccggccgcca tgtcgacgca
     5401 ctcctcgatc ggcacatggg cccgccgctc gaacgccacg tcctgcacct gcccgcggat
     5461 gaggtcgcgg gtggtgcggg cgagcagccg cgccgcggcc gcccccgtgg gcagcccgca
     5521 gtcgagcagc acctcctgcg cgagcgccag cagcgcgtca ccggtgagga tcgcgcccga
     5581 ggcgccccac agcgtccaga ccgtacgtcg atgccgacgc tcctcgtcgc cgtccatcag
     5641 gtcgtcgtgc agcaacgaga agttgtgcac cagttcgacg gccaccgcac cgggcaggcc
     5701 ggtttccgcc ggtgcgccgg ccgcctgcgc cgacagcagc gcgagcgcgg gccgtacggc
     5761 cttgcctccg ccgccgtccg ccggggtgcc gtcggcctcg atccagccga ggtggtacgc
     5821 cgcctgatgg cgggtctcct cgtcgagcag gtggagcgcg gtgcgcaacg ccggtgtgat
     5881 cgcgtcgcgg ctgcggtgca gactttccgg ggtcacggtc atatgtactc agtagtcctt
     5941 cgtctcgata aaacgctctg gagcccgggg gttcgcgtcc tgacctccga cccacgcggt
     6001 cgggtgtgtc gcgctcttgc tgacagagat atccgatatg gatttcccta ccgagggaca
     6061 cggcccgaaa aaaaccgcag gaccgggggc acaacaccca ccgggcgcct ggtcggcacc
     6121 ccgctgaaac ttcccggaag attcccgtac gtccgcccca gcgctgcctt tcgacagatc
     6181 gggcgacgag tactgtggga ctcgcttcca gtcctcccgg cgggaggcgc acagcatcgg
     6241 cactcgaccg agcaactccg acagtctgcc atacggggca cgggcctggc caatcgggcc
     6301 gaggatcgta ccccgagggt gacgcaggtc aaaccccggg cactcagcaa gggtcgcaca
     6361 ccgatccgcc cggggcacac gcgtccgacc ccggtgccac cggccggccg ggaacgtgag
     6421 cacatgtgct catcccggca gcccccttcc gggtgaacag aacatgtccg tggaattgga
     6481 cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt
     6541 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     6601 atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     6661 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagcaa cgatgttacg
     6721 cagcagggca gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat cattcgcaca
     6781 tgtaggctcg gccctgacca agtcaaatcc atgcgggctg ctcttgatct tttcggtcgt
     6841 gagttcggag acgtagccac ctactcccaa catcagccgg actccgatta cctcgggaac
     6901 ttgctccgta gtaagacatt catcgcgctt gctgccttcg accaagaagc ggttgttggc
     6961 gctctcgcgg cttacgttct gcccaggttt gagcagccgc gtagtgagat ctatatctat
     7021 gatctcgcag tctccggcga gcaccggagg cagggcattg ccaccgcgct catcaatctc
     7081 ctcaagcatg aggccaacgc gcttggtgct tatgtgatct acgtgcaagc agattacggt
     7141 gacgatcccg cagtggctct ctatacaaag ttgggcatac gggaagaagt gatgcacttt
     7201 gatatcgacc caagtaccgc cacctaacaa ttcgttcaag ccgagatcgc tagc
//
